	   SnapGene      îGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCGCGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCGACAATTGCATGAAGAATCTGCTTAGGGTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATACGCGTTGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCTCTGGCTAACTAGAGAACCCACTGCTTACTGGCTTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTGGCTAGCGTTTAAACTTAAGCTTGGTACCGAGCTCGGATCCACTAGTCCAGTGTGGTGGAATTCgccaccatggcggcgatggcggtcgggggtgctggtgggagccgcgtgtccagcgggagggacctgaattgcgtccccgaaatagctgacacactaggggctgtggccaagcaggggtttgatttcctctgcatgcctgtcttccatccgcgtttcaagagggagttcattcaggaacctgctaagaatcggcccggtccccagacacgatcagacctactgctgtcaggaagggactggaatacgctaattgtgggaaagctttctccatggattcgtccagactcaaaagtggagaagattcgcaggaactccgaggcggccatgttacaggagctgaattttggtgcatatttgggtcttccagctttcctgctgccccttaatcaggaagataacaccaacctggccagagttttgaccaaccacatccacactggccatcactcttccatgttctggatgcgggtacccttggtggcaccagaggacctgagagatgatataattgagaatgcaccaactacacacacagaggagtacagtggggaggagaaaacgtggatgtggtggcacaacttccggactttgtgtgactatagtaagaggattgcagtggctcttgaaattggggctgacctcccatctaatcatgtcattgatcgctggcttggggagcccatcaaagcagccattctccccactagcattttcctgaccaataagaagggatttcctgttctttctaagatgcaccagaggctcatcttccggctcctcaagttggaggtgcagttcatcatcacaggcaccaaccaccactcagagaaggagttctgctcctacctccaatacctggaatacttaagccagaaccgtcctccacctaatgcctatgaactctttgccaagggctatgaagactatctgcagtccccgcttcagccactgatggacaatctggaatctcagacatatgaagtgtttgaaaaggaccccatcaaatactctcagtaccagcaggccatctataaatgtctgctagaccgagtaccagaagaggagaaggataccaatgtccaggtactgatggtgctgggagcaggacggggacccctggtgaacgcttccctgcgggcagccaagcaggccgaccggcggataaagctgtatgctgtggagaaaaacccaaatgccgtggtgacgctagagaactggcagtttgaagaatggggaagccaagtgaccgtagtctcatcagacatgagggaatgggtggctccagagaaagcagacatcattgtcagtgagcttctgggctcatttgctgacaatgaattgtcgcctgagtgcctggatggagcccagcacttcctaaaagatgatggtgtgagcatccccggggagtacacttcctttctggctcccatctcttcctccaagctgtacaatgaggtccgagcctgtagggagaaggaccgtgaccctgaggcccagtttgagatgccttatgtggtacggctgcacaacttccaccagctctctgcaccccagccctgtttcaccttcagccatcccaacagagatcctatgattgacaacaaccgctattgcaccttggaatttcctgtggaggtgaacacagtactacatggctttgccggctactttgagactgtgctttatcaggacatcactctgagtatccgtccagagactcactctcctgggatgttctcatggtttcccatcctcttccctattaagcagcccataacggtacgtgaaggccaaaccatctgtgtgcgtttctggcgatgcagcaattccaagaaggtgtggtatgagtgggctgtgacagcaccagtctgttctgctattcataaccccacaggccgctcatataccattggcctcCTCGAGGAACAAAAACTAATATCAGAGGAAGATTTGGAACAGAAACTAATCAGTGAAGAAGATTTAGAGCAAAAGTTAATTTCCGAGGAGGACTTAtgaGGGCCCGTTTAAACCCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGCTTCTGAGGCGGAAAGAACCAGCTGGGGCTCTAGGGGGTATCCCCACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTAATTCTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTCCCGGGAGCTTGTATATCCATTTTCGGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGACCGACCAAGCGACGCCCAACCTGCCATCACGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCGTTTTCCGGGACGCCGGCTGGATGATCCTCCAGCGCGGGGATCTCATGCTGGAGTTCTTCGCCCACCCCAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGTATACCGTCGACCTCTAGCTAGAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCGACGGATCGGGAGATCTCCCGATCCCCTATGGT  ?Ý   $   +         #   )   $      '            &      5       &            +                                 "   -                                  $               !            -            1      (   1      )      (      '      '      #               !         *   #               $   "   8         $                        $   !                     $      !         *   $                                              !         %   '   #   0   "         (   #               !   $   (                                                    !   #                      #      *   ,         (   3               $      %      *                 (   #   $                               
               )   *   &   #       -      !                  .               -   '   %   (                      '   %      *       +            .   1   %   *       *               .      ,        $ ñ y´µü¾<ðk	)*+05DEf”�žBCl×–´!£T�¢+ yzœ¸úNÀÒÖ<„š¿=tåñl�	X	\
FOgµóøy—ô"¤¡yIèS Ô – ò �¶ n•,1ŸÈP‚ojµUúh£ · `æý+ÀÕ
|6q•D•5mÉØ±e7éM©‘(# C “�ûOÁÓ=Áè´ÀÃ>­ÚÁæ6ë	Y	û	oG¾Œ˜¶¤#JÕ) •I€Š›Ï!õ^m�ï	3<ØÿP†æ^u½ôª@lžõ¯ÔZ¢×e¥é‚	!1$ f{«hÎûuÇ#ç]
JhËÝãþ¦¿S…˜¥‡`4z•T¡{Ï z¹×…„ãU|ìò_	4
r;Ô¥ùÈsz<' �D÷’·½õxÔ°ßúK–	þ	Ç
Ü-2nÉÎƒp¸‘=k¶+Ñz�.
û $žåêsoÖ†	Ž
Ì
 ËuŠÄ4Yo¶ãA — Lmi
ÑQ‰©,
uaÏ+’
V‹Êi¤ F ó þ°_J	ý±``×V�Ýº˜& œ açî¤§­Ù ±
ƒ·ÅnÊ	Xû#p§ªU(8n‡í.Mªwf2H’) o ®õR©{×Ã\7^f�Õ6«Lòü&Ð5 t 40\þ7” ,™Á)	‹	

Ö
rz–)E�ÉD�¡#+F_³¼þJ[nÙôf‹²f�hûêžNƒF  ¸ +ìZÜ3Ršn	˜
}–$ag›ÀGbÄÏá“Zªµ& D üÔ:×‚²Áù’®¿�H	l	ü	1mpzýH�_eŠ�™á!©&)múK PÂŠŒšÄÕÂ7U Z	x	Ê	#
M
×9@$‡s#±Ö M ”ž>8µÛ´ß	@
Ñ
¿þ¥À)ƒÞÓñi9 X yÂé?ç¸ì	O	[7w·–šÏ¡O™Äq¼i®þw+ ˆ �Ä"q×2n¼Ädn\	•
Ä
4ORÙQ‘Ù‚îõ«ÌžŸ[£‹¨Ç=¶¦µê«
[ 8êœÆw9¢B ‡v¾;‰A]uö°1Ø,jƒ•2’¸ ‹ –ÝU‹îœö_g‘Óð	Q_™4­m�%":‡  JÐýW
=‹çõ�ç´Õö«fk  ° GªiŽÏ‰RÜè 		z	^
´
i†ÞûON§À&†ù½ ½ g%‘È$¢•¦Fˆ;DkU| Ï |¬ÇüvÔ	7UKW}
<ñÿTÚBHa!{‰Š €l0Ì	�ÌÑäiºÃ‰™í5@H–¢`r ¢³×ÐØà Q]†Ùâ“ÝíN¦¸¦ÆâÉû,{	[ÆÌ } É ógVgˆ©ÍØ	5
Ü
<TÙ?€89â, Ö {rºC†¶äî5%
O
s™ÕH1ú\R=>+" y 8CRØq?…h}°óx`	f	Òàn„ˆ~t¶ $Cwu5- ‘dø�“¾˜¿y¼ÌÒÕ4I—„	ù	Ê
Ó
.3oÌÏ“Ò\s»º>`n,¨Ž‘c¸, EìûLv¶		d	ÿ	“
Ú
MÞ„ˆ“sÂºøü < > »ñŽÉêq	¥
È
Êceq¹Xlq©Òî6/¼* R ¸ö±àá)
§
�ÇÝ]’ÜÂ· fð{avË½ O ë|U	²�…ÁÔtä8÷ å %mŸæÊõX«‹Ú5ZÕó®õÀ + `–½--B
Í
¡”Ü½?Ì)92vÅp°·ËB,‹ 	 ³ ²Ë#Vótp·‘¶×	A	‡	
�
  @ Æ ª§µ»åˆbÙçà$â|¤²;®7 " ˜ Û âcÒ-kÐ{“ÎtækŒ¾Ëj  Ÿ Mn ¤c3:s`t	²	j
«
‚¸ªèr¼j,ðP¹	=ô$ û d@c~¶Ýa
ê
"RŠÇÜ-ZvÍì §drÍÛÿ…úW‰¹¥ G Ç±Ø·@ñýaØ3:É ô T‰Ü3–|*²3g­õcè#a%‚1§éK …ÿ&¨æ
þ²5$WÞ×p ‰Ëä`±	@ÑK	H
ëÁzÂ��+»™! Jkè¥®ÐÚl	!l„µÃÆ±ñVYq¨
”)lsˆx‰0y V ® ¨²
O
‡˜$«*o/k	qg3“* � Ä bïaÇâ1©
ÜË†V×0åîN¦Iò T ¤ «¸Á×ñùgo¯ÔÞüþâ’Ü9Zh«½'°^ª- ¯ S	
Ì K³=—é]¦‚
·
8[ÙŠŽÒÖ\i¢¬ÖßÃ¬MPf)²¹Ñh Z p àº
]@˜±àù¨QÜ¾Éóf“À�   „ öø^	_º�¡åéí_‘Ñ äâù¢	'Ùß^   ª| ØŒÄó�	¥	è	à
g7œòýÔÍ¬1 J ¦ 5Yºðÿyë4â*1Ê>	Œ	×
Œc‘9EG`½ÙÿK\g›³‚¡gWiê¾æëÄc« e 9]P‘-¬Qâ	
ï
äy$,´oxŒKÆ}Ÿí ( 8 u ‘ © )1o 
8Fº
+
{*_�¢¹e-K£ºÄgÆQéC€­ïOGü1  1 {•¡âšÂ*	ž	s—ÿ8F‚Ê¯JÞ0ÚõÔ‚Þ;K\|ü^j¸ß¿ç^|„6Q�>x  ÄF
&bhRU6®ä1HcÑQ~›â}¢«å¶) ' ¹ rÆœÝ4SN &L›q¾	™
ª®ßí—Áàð8ÅÐFSÊžþî`¿ 0ñ"ÛãQ	}	”ŸÉ%kqœ)éóT°¹“”'Ì( ,<J¦ÏíýÕ[Aîo	­	…
~¼­óû�±(2=q”v/®å[D • xMÕû;ƒs*ŽW	{CœO�nk÷Ö¢xL¨'g¡' HÎç ƒ³ÂúÙ{ÀÆë^	3
2nqþ:I‹Üâ¤¾~®Ó;_3”  E ý¯ÕI	ý	Æ
Û·	`*ÃÖn' n s › íØ“Â™m	—
 ]‚€ŒÈÔ`fŽš "ªóú'†ÃÎàúLvû%e 97�‹±ø‘Ô¾TŸÉ	L
0Z^Ö Î N%(h‚Ýð# Š TÃ›ÅpÖ»Ã8Vf¡[	Ã
AQŠ�Ø�:œ³§ª+<´ª$j‘ ¼ Æ$�ˆÿy	Ë	6TúAE ?ˆ¡:‰ø²× Qfpµ4f¯‡¨$
N
S¥Ø>ƒ8t Q º�¾»puµàÒ
L\’Ñ‡¨Á�b÷`·»)u¼  * N ä l•±Ê"U¶ô�µ	
A
“Û„>¯ÊÔòö­! ! Ú ú Ÿ?9©µÜ_s	b¦j,ÿ¦Ì„Íß¸å#ùj±:¸ 2„ˆ?~å
Ày¬Áôð$*Ö j`Ï´ÀÛðøf®ð†—…á	)‘r¥j¯ˆñx* Y 	J]–è¥ò	�
¹
ß
\?Z‰œ°Ñäø›ÐÛáø¡½ÈOe’ÓgÞÿ]# d XnzïŸê	)¹=	�	�78x®¸Ø¢ÅJPfÅ{]·åæìP� & Ã@MÿKpíP	¬	½	„
“­­ãš¯RÉ�¡ m r ‚rØ)zÅ]	–
Å
ÚBöù_…ÂÍß2w“§dŸ& ‰ 8SÅ#÷o‡3½eþÂ
5PSR‰Õù‚ÍŸ²D7©Ü;>÷ ¹Éƒ½¨otax’ÀÚƒ=§#Œ·É¶õ¬°t»$ % c WmžIþ(o¤<	€
¿6›ã­ï…¬(Ú÷ ®\¤äÈë‡\" �¼Hy'£ý`ˆwš¿â¬<^Ùö¦­„±ÁÌÞCÛ1’È†sº8 \ ¯Â»Çx:;ü#$_øtuv012DEª«BÀËÜÝÙÚ-./0k�‘°±ƒ„…r„–“”¹ ] 'è°Ãâ9ë†¤Bù=3FCÕéòv«²¾3•� ¡gÈ<œ
%C†wáŠ÷2õùl×˜…—™$ (ZŒßé]™Äã�¥ÎCh’ÐE	ú4`šSD®Ì&½³ìh4ˆ á Wu>ìŒlî÷‡*õWÎ5ÖÞó�•&NX#;R–ž ^ € ë :—Þ”ï–Ôñ	¼
fGRV¨ðLêwˆÓ¬ÞÝ  Œ ç ±V$Y`H>BnZž¿Ãó @oµÉþ‹
L©g]† j Ì(Æ	Û	
 >èëAö;‹’µ\¬öš ¢ÏhÑ¥ÜX“	&Dxâ"žè·É˜mØ>Âl˜Ó !K.CÉ=Þ	�
ø
‡Œò³û3øÖ3÷/ú™Â$ 
)�êB^šä¢«©SÝi“é	
g
x‡BüH¨!;E¯Í^DÈÎè! † [à�0l‹Ñ	F	Ò	Ï
xû5ßï/›Á[‡¾…´Ñipu ± bÅàaÐ(¦Ï‚	_
a'3¸rz¾5‰  ÄûH jžŠ/D{	µ
aj–ŸÞçZPOT'Ÿíìú.  â ÿ jvhKíòX£Ïÿƒßdøµ!'eŸ X&”m¢%‰
ö
öÊ×ô¤§A<–ÛV$— ¾ Âú’j	+¿6–ÍÏS� À ?�ïøˆÉa;•ŽG‰ElOY<B}$ � Ì Ó NƒÖ�Úß•—ý-¯ÕÕ	å	L)ò ˜ÀxÔ4ßXm Ð Þ ì ÷ ø/<;}
¼~S©ñÐb"Š­ÛU! _ ˜}ðü`wÆò	8VgX=HMVÅCëû²|‹`ƒ�° ˜D­ÈQ½
ò
$W6|'zUÛI	‰LÞ 65W1?šåC·Ž“YÎæðo!C)¡Ñ* è ¶¿Z²mV%Eº½ƒ„¬0
8
-I�ÒE»Ó8{;ä#0[xŸ�Àô�aÁ|d$  - � ' ™9Zaž0	¡
±ø£ëÍ&Äš+Ç¹6I—ÄV£ØáÙ �-I	Á	Í	D
q
£ÁAjŠ~–îA£ÈÒsñ´á µ ï "œãw¶õî‰	Œ
d§Ç	É�œRGQ/Í AAj§ÄÊü>—
ô<hB^ R^‡Ú+”Ê‘
 Ž¹Åãžr‡Ç Â pÍÞ�ÿO©ÕMc-|ª¼%\z4 å¯¹ÀÇ	
Ý
þ
?=Ú<š9]€­ã#  h k ~ ÍÚWÎC	‘	Ù	Ü	!UéB÷9@Óçð†�÷› Ê ´%XGh‰ª
6
Œ�“Î¶nc® ôöh)M©«­	
çìŽ:¢- £&-v‡OOÙY&
šèã2Ÿëûé]âOIVÖæÔ × H…ø¦ïZ6

'EI>™¤? |ª»iÝåúõ	”	
téy¸?ÙÃmè   sÐD·ÒYÐŠ;
P
T
âÖî#¢ÊS€n,Ä™Ç>D z 9v±ya	ß	á…u·!N=×„	þn€6[¨! ?¬Ÿ+@D~¢iŽÆÊÍ“¸Ëš	ž
â
�´ü‚%IûéSšî 	ÙLr/†èñ·	ê	i‰+r4Dù‹Ñ�ÿÂ D‹"Sr~ô>g	§	ù
ˆÓoó�²4xø0vÃÏ% Ž_›åù¿™ÿ½;äj”¿[
Ô
(/4Nep“”4i°d³6èù' B ’e”‰ªÀgÍ¬ôT]5æêî	ú	;×½Óå]t¼—ý©Ç?R„£Yd�# Cr‘¼ôwÓÞùJ	…	h
Ë
ÍÐˆ+<Î_X�µâ€Ü¹-@É	é0 *3­ëôQ¨/[£¬2QzÖ6[˜(	
°
yC»¿ýI"FaoŠ¦Tì-©K’YE´Ï" L ‰™Œ·Þ
	G	k	w	"
?
Ð
yü€6v‰à¿Ã»(†Òù"°ýv ‡ Üí1mvücÒ 
”
Nô˜œí�\tŠÆµ9Z†  Î F�/QÛ	Ó	Ž|…¡	;ÐðNMhÂ”ˆìGjqÖ( x È Õ 7P\…Øáq’Üò>ígMwÌe	×	Û
Ñß·G0µúQZá*Å# ; = �—Ñéë3	ƒ	ø	(
¤
¦
É
Ù
œË[bdrºÛ_mpr¹íï~ ² ¼ò,@	T	±¼(8ªÀÓs7+¿Š ? c£áb}r±	`
é
´·ÛYë»izqá{½­6 ÆÊ%{)§Ð±×êfb~Œç"¶4üŽ0¦èo S £ Iák¨
kÓUÖÛ/Ykg¼/! ƒ ÷Ÿÿ‹²<Ž	¤	ç	¶
— ßè[§¡P^Âñe¿€ØÌ$  0 7 � ¨ (¹Oxá«á0É*
î
b^IÝ/,šÃ é|Ãîbû=w(  ¥Åü!%Eâ|	
ž©»ÈÞìpQ“5u(07¸ä|&äíC¾ ” š wìLúV	›N€mŸùÂÕu  ®SY¤M¦ 9ï�   P Ù ã ù k1‡ó}¥[‘Ð«ó †"ø(  i_î\Y³÷„àIe¶dåŽf $ b lRön†ý
¾6vô¯ö% & çf£&û…÷</Øõ¥¬ÛBª¯½ÇÖ—q˜ à æ Y=“•#)GeôÍUKËY¼ÒŒòÝÜW nË'Š
÷
@K!È¨[—=Á 	aÃú/k.�	Ñ	Î
ww•ÝY.ZqÐCÍ-ç ¿ J“ñ¡
¾É:@ÌÎ Ò Ý —Õ.;ûä	|
ñ
#(U{&—¿3±ÚTWl‚Œ  , ¾48/
C
,¢÷À¶@7zÍï*/}w•Æ B¸ŒŽÇÀc ´ Á î !›Ì*ô@Žˆ	�
ÔcbÈ–�›$QAFP 
 g ³$Wä¨¸	B	
æÒ¡"m~ ,uù‰Ø	

¡åç=C >uqçð’·ÊqŽ<HƒŠÐèRmZ§! A ¬P§qóþv:«í	:M¼*–‰¥cS5´áÛ?³€# w Ç Í Ô O„×p‘Û=˜
.ubv	Ö	!
Ð{¶ógù'‰ëF¯8Y… : ¢–è°Ö¶Ú»}æµºyà}¥n  ‚ ¢ àþ$Èæ	
¨ºë¦Ò./™ÁÕÚ.è%ã<B “ ™ ø 0XMZlªòc�� # a ß <ekü~
d„½Ì.?JTÑu©®¼ñÜp Ñ Ü ù3€	.
vTX¾È.|”A‹�Æ¿ÌVbk í  š=+ãÓbÇÑ‚Ï#çQlä¦æ# ¡ Oo¡¦òý<ç#taÇu	
 
9¹µ•¥|-³E~
>„ ` ™d-
WƒLYaË>ISWÆ½¨»ŒÅoåaŽ*   N¥ñ;a«¬NOPQR’“”¤Æz{MsüýþÿñòL}§ºþäLM, ~~½4¢bx‚$	³	ó	k
¬
hï
,­ ÇéNDtì këóQ–¯MÿêõN± « ™®É#®!Èê({íÜEi«ežÎÜÔû•�º ü 5AE·H´	º	ó
‹Ví šIMÛëO( £dRƒÞ¼%	b
­
ë
¢ %}ÈX7wOìöW¨
—°†Nß Šö3 e¾)]ßcyœ÷ 	5	–	ô	l
w
¾
iÌð-SÝ.}[Î¼JulŠì?EôRsXH¦² }²X@û"É)D¤jò^fŸÏÕ–ŽÒ 6—là|”¸ZFA»Ê È¯æ¸ÀpþbdÙ"4*8;¢É H ¬ 7šÊ2Ù	$›¯ë�ÜîÏÝçñ¬IUÝDü$  Fn}ÒIdŽ…³»	1
.J‘h®öd¼Ô|y &Ê`õ�N¨Üe é UŠ·Ý[—³6¹F¦„4ð¦îÓ€÷$‚!9ŽÁbL�P% õ ý À4B&+—¸»‚­Äe
ô
$F9W�!1\b³ƒJÆêz}@s uFW¾µ	9
R
Œé)<åî„ÌàM~ºp›~2Âì*   . Ž eß:[©½&	¢	=
¢
®
ç
ì
²&~YntÎLÅP6ºÁXßW ÷ ( !„Ÿ£!u™:ÿíXGOÅØ  '[€š!3	u
ÉØx³÷©,=%‡éJ˜â@‹¤ÙâF   †¤SbŒ€1	 	V
c
ù¤ì8'›l˜±È7à›qÚ Ò.Ôà�L	E
jT%/:\Žkv@þt�ïÉS�ò§( €€ˆ¿‚ŠÌ*¦²J^ëÒœ9	—	I
¿
ÂÞ~ÂBKŽ‹í—õá,†ÏÓµ# dz£ä
ø!	6	Î	õ	X
r
ÍìñÏ2½‹mFnB¤¼’šI$ 3 fåaUA	,	Â	m
x
Áä¤.H{„ÃÌ±ÖS`š.Yt³Üâ ¶ ð x·³Ñmï
	ÇA´Èòx•ó  SHçR�Ó ~â"…Ê¥rk�†Ö¤0zÎ #�äKléö¯^�
m¨üt�°Ê*_t‰‘Ð3y	UŠ—  ¦YÛŸŠ	
¶Ä(e#*E^²WZm
e©€gm�ñ‚1G W xÖ³N	ld¨8•™ý?˜plr­h8 7éPˆËæ3¬0õi”· ¯ ©k³
V¹%ÙG
C_Ÿ¼ß | BB˜m‡Åá}[#+B7v=4+Ë cðÈËHc	’
ÆÝW¹’Áÿe§5+Ê
 _,É{�ŸW1Ùô ž Å b2ª
�!ºÆçÌä‡qØcæïOÚþs<£ˆó SˆÛ•ã°G
2?#29JÈ U ­ Ã Æ0ªÂÖN°Ýý“ä&p]©  ßË
¹ØìhÕ�ÿÞã«(±¸«) I ¥ 8�3E Pá	‹ãþdJw¹Ó�Ýè:BJV[i¬½Þ¾ ]{5ª* /;IqÎðÔ›Ú¬òú%œ°'1<jTpßïÐèò.PS}­ ’EËý_& G~ÍæÁ˜Ôê2
ÿ
9\‹¶ÇÓÛj£½Õò}¡­Ò:K^$f# » 
eo´6€°�Óeº†ž§È	K
/@�×]€=›'g�*ˆ³©i   ) ° !TŒº´>�^´r	
ä
Ki+ïË®Ìaäö_Õ º·- ÎLJ•ñœ	«	¼	ƒ
¸
Þ
’¬>�Úïˆ¯Ð÷e­×âà�ÄzÇNQ‘œ OÒÝð l q ¸È‚V7§(s;	Á
A5Ôø�„ø'"ã:cž! [ ®ÁáºG…A›
"^sàC™á©Ôèñƒ¿Êôø�‚œ   ê Vt‹Þ\˜kíXÍÏD	»
V§ïARÝ%‡Âëg”MQ i ?´Î-B¤ÛÈ’	Å	Ú	Ý	êñ²ú:2¶‘.Á\Ò … A¡¨ß`'Š
f
`žæAîG :ž2]·„oyÇët  þ iÁùiˆ
õ
þ`‚c”£÷´ ÚdA€ Ë ö M‚÷�ÙC,P_®Å»5ÄyúÏ
K~_¯. µ&5Y,HUD˜¹¼‚�ƒ«	/	À	7
p
 
°™¢ê%D�’ÒXå:ã"UÑ(ð{à vi¨�ÄÃ�=»ó;qy3. õFLª¬®Ù¿�	
ý
8æï…��Í™b­  ©%G„÷NXYÏNô	:
S
áçèíêáNHU£ÃÕÆ Š«ž*}¡�ÅÌ™	¦	¶	é	á
h*�M±ýœþÎíÁ- 2ó.Z»ˆ1Pf5ó\ãøZ¾å'	Z
¯
Ö'd’ä3¾üÆQhƒ¢²ëXçJWXø'  K 6ÛñìˆfPÚËÝj	>
� :FuÏM—ìLœ´PÅYà)!ÕÄ%  $z|+2?	S	°	÷	'
£
Ø
è
›³é°Za‹Ú!ê^7hoûp�¿ìç¬(  / 6 � § ¤'Nwà;ª�	£	í
oH  4]t+6ãXj{»Âd¾× Ø ^†ù[­R¤šöžHL¥Ÿî Q’"…ú(Fó; J»Ç–Æ  Ô:Ið.7 Ð	ã	{
ð
"vö 'z%-µyîY?Ùæ f )t§æ

åp�~š P@! 9 v «pu	9ì	ÏêzÙºÑ-f´.¤ø¹R&ˆêÚA ’ *2jû	uÇ-�@ðPã¥' }¼¡æ�"#	

,
î	+K`´Ê‘£yLÅê•®DK¦ÄýnãéK% ª (\Þ›G»ö	4	•	¹	v
¡Ë|»ëõVhª>D�Ó™H”ŒÚG |–kúß£Û¿HT]c7È*  tE¸–¥Ñc��ÃQ
d
ï¥í(öœ ƒËßL}� ²¹É8_o}Åyœ?r    Z ‹2	Ÿ	¡	<
U
t
t˜9×msKF<kÀèá?šE+ 2 ‡Ñ¥ÓT¢ãê›+	8	W
Àã GƒË$9°1Õ�ý_m™à-…ÎR‘œÛ ]áž	
'³]s�l¯dwœ…2ð�£æTF { wèÕ²M	kUc†7å2Ø"6u¬ž7¶Þ Gzb	F
€Å1V‘>Øý1ó.  .:HïÓÅ/Dà	Šâý&0;iSIv¸ÒOR€çAãÿ~‘
ÿoÊ·\©¨1 ( º ÿ‹Íd³5�”¹Ó�ðq	›	ª	J
ã
«Žî~‡µÆÏ*áñ�îÆÑJË^‡›ŸÔÿ¶ï% >sÇ�­ÀU,A£jÇì'Ì@r:	Ä	š
À
rßƒ&1µâ-ê[Ñ* Lh�@ŽØ +§ÞT_CO�œ.	¿	o
‡
Ÿ
¯˜ý4@ÃCx9öTÐxï  ¨‰$ƒ�EKXËÎM˜	ü
àæ§ÃÂ€L°Œ˜GTüxaÅÀ* 5 1òÚ#M0‡e{ò÷ÜäR	i	¯	ö	Y
Õ¯ŸŠ– 3s*5n±úWozŒ(½Ã ÓsåùÏ	z
òõõ&
�º•ž å 
t"	~	¸	ë	 ÊÎíJ³Ð,êôU”œÒC¥“J ¤é7	s
•¿jlr¾ŒEGbÍ› -Gîç\C	‰Äü0²r�×æ5ü10EµÝ. =Kg§þ�×#TcBW€?ï-	p	©	Ã	n
†
û
å¦q}†ÅÎ)3?w0¯´]wZwÄÐ 4 Òsöh	®	y
¾Ô®ôIž²ô	•Vay›ä, EFÐÑŒÖæbVBõ	¨	ú
½ÂÃ¥/p|…ÄÍ>³TUùúû/\uv´ã  1¾  ›GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                  .                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
                	                                                             
                                        	         #                                                                                                             	         '                                                                                    $                                        	                   
              
   
   
                           
                     /            
      	   	      
   	            	            	               
                           
            	               .   !      #                      ‘   `      "   w   o         &      ]                                                                                                                                                                       .                                                                                                                                                                                                                                                                                               
      	                       
              	   #                                       	   '                              $              	       
      
 
 
         
       /    
  	 	  
 	    	    	     
         
    	     . !  #        ‘ `  " w o   &  ]   z¿  |  |!  |W  €ß  †M  Ž«©¸„_ìì�Í_¤ì®ô;Vv�ÆØ~•+ŽÖu­¾) æJÔQÓâœ×QƒÈ4Ã,¦íœ¢ÂxÛM
eÞÞP
Ã­¾#˜à� ,¦ÆíJbœ¥Ç8Œ=!
©
×
c{¸ºäÿ˜àbh`´ËV\Ñõ$/°Ü0så¢‹\A¬RO
+uyR@
9+R+yÌA 
 (Ç.~ÆÑf®Õ¿±Ããòm E0ÑºÃÐéÜ0c‚Èi	‘ ;Ìžr´Öƒ{ñ{lÌ}ÉÎ�ñ=–×vÖƒ{ñÈšQ†Ù“Æ9@ÉàÓLr4so
©so{ÃÜfn‘¶üýÒ€qw†Û2Q¿Fa´] –îð	FéìœÅG¹	ÚQ†Ù“n,{HÆjûÂ ¶× …yÜšðvËëðËë	²|Þ ;SÐÛ�]|5’ðËë2Q¿y?Àì|q•DØ=¿cÈn2Q&_�å B
¡?Ç‡{ŽÐ2þp	)]Ið;S	° ¿~!ðøn8ª×©sÁÖI_o{> Ã|ñò™ë)	|ÜEQf�Én‘¶ÔûüZƒV¤¢›�…&¥ö 8œŸPs*�
��@ §Õt'öG			ë	Y
kÔ}Å@c�²±VœàŽ„SQf
&®c~€ü²Á¿mŠ¢ï©	†Îâc‘gæ±�	£	¯_Ÿ4@ §@¬ y?Àì|q•D5’ÜšØðvËë@	ƒs¶9@ §t'ë	@�Z2	t
<E¼ÌžV ,íY]4—	
ª[_íû&Š�Òå½˜Úàü )-c~åoÅêž@ §G@_�å ¶*Ë	Ë˜4?•¡¡²×® ¨²
$5™�å ¹%áÕ	²dƒ¨»o ƒpÒ%/:þ�Éyìœ¢ÅG›¹	�…Ú•ˆÐNwÂ–ŸçÁiÆ @ÉmžÆ Ÿñû	ÿ	ŠÁÒ9@ðÕËÉë¬ 6F}²eàd[œ1
I
~Ó€¼ÅP|Ö÷<òŸ%‡é9ƒ†sÒÜ Â t,@ ª»
&c~ÎŠ�×
ÿ©b6«; �×@Éj¥ûö &8œR¸y °„ 5û^– ±"U¶	@	
[Ð+ŠÎÉËã˜4•¡Ï€Ì	i‰í@¢r³à5
ubó‰8OY…1ò0òÓ÷äY
Õ~µÆñ±ÑJW…pP#
M
×s¦ KŒ	‚
·
ÙG¬Öÿ\ßÃWiMPcÑ· +ì×Z_„|òJ	_	n	|}•sZ©º˜\		²³rw1æEDúKþ	ƒ
ûµ	)›~ì×_„|òJ	_	sº˜K‚
·
Ù¬ÖßÃMPÑ¦ Œ	Gÿ\Wic+TC�œ.	¿	o
Ÿ
¯˜C9TÐïªRÜè	†û§‰	d	œFU ¦ ¥ÄÚKŒ	‚
·
µÃÙhGR¬±ÖÿVY\H¨ÑßQÃWilâ}MPc¶Ñ01ò\0òÓ÷ä		Y
Õ~µÆ²³rñw±ÑJW1æE	¡é‘Ñäâ'ß Gi‰z	´
iON&ù-‡OYã2ûIV( ÿ‹q	ã
*îË^Ôÿ¶hÎç]
hÝ¦¿…,H	À	p
Ñð Ñc�É_K ˆÝj	>
u!y¸Ö„ñøyä`@zÂ"œãõ¹ya	
&
Œ
šè§é]É=�×­ãæ K ’ Qˆ…Ñc�Ýj	>
uÉ_!Œº´Kaö_º%NNçêáNHUÕÝN,{[âI-GîC‰üæÐl	Æñ
”‹ rCU¬¶Ç_O
¿ÕTÚHmR�)+Åá+?29ÈË }nÔÒd…L	=
.j~TtÉÎL¤j&tÕXß�¨ ¸	 ÊêôUœÒ“�q×Ä\	•
Ä
Ùõ¦
1 ¡âš*	ÿF‚Ê¯0Ôü^ß„Q�Áè>æë¶mÊâìLvd	Ú
ÒÞèÐZÎæ1kÀˆ Ä"2¼d4ORQÌž¨='[š3	u
©=˜‹ÙF¿)rC¶O
ÕR+ìLvd	Ú
Þè1‹ U¬Ç_TÚHm� ¥ÄÚµÃhR±VYH¨ÑQlâ}¶0%ŒNºN´KçêáNHUaö_ºÕ† 0lÑ›[´ü A·ó
VI/ÍE
òU ­ Ã Æ0ªÂÖN°Ýý“ä&p]© .:HïÓÅ/Dà	Šâý&0;iSIv¸ÒOR€çAãÿ~‘
ÿoÊ·\©¨Jkè¥®ÐÚl	!l„µÃÆ±ñVYq¨
”)lsˆx‰0y	 ³ ²Ë#Vótp·‘¶×	A	‡	
�
 d XnzïŸê	)¹=	�	�78x®¸Ø¢ÅJPfÅ{]·åæìP�T ¤ «¸Á×ñùgo¯ÔÞüþâ’Ü9Zh«½'°^ªØ ^†ù[­R¤šöžHL¥Ÿî€l0Ì	�ÌÑäiºÃ‰™í5@H–¢`r ¢³×ÐØà¸ +ìZÜ3Ršn	˜
}–$ag›ÀGbÄÏá“Zªµ]áž	
'³]s�l¯dwœ…2ð�£æTF… A¡¨ß`'Š
f
`žæAîG :ž2]·„oyÇët& d t ¸ +4ì0X\nzÃïþZŸÜê	3R7@M” ÿ,Kš)p™¹Áí)	=	P	n	‹	�	¬	½	

„
˜
Ö
}“­rz�–)7E–$8�ÉDagx�›¡®¸#+F_³¼ÀØþJ[n­ÙãôGbf‹¢²f�ÅJPfš¯Åh{ÄÏáû]·åêR“žÉæNZƒ�¡ªìFP�µ … ¿ þ 	aiÁÃúAJ¡¨ßù`“ñ'/ik¡.Š�	Ñ	
f
ˆ
Î
õ

w`w•žÝæþAY¾î.G` :‚ZžÉ2:]c”£·÷@´q„ÌÎÐ CoyÇÍÚë-dAt€çR^‡Ú+”Ê‘
 Ž¹Åãžr‡Çƒ ÷Ÿÿ‹²<Ž	¤	ç	¶
— ßè[§¡P^Âñe¿€ØÌì( 5 º 1òÿ‹ÍÚ#M]d³05‡áe{�žò”¹Ó÷�Üäð	R	i	q	›	ª	¯	ö	
J
Y
ã
«ÕŽ¯î'Ÿ~‡µÆÏ*³]sŠ�– 3ls¯áñ*d5w�nœ±îúÆÑJWoz…Œ2Ëð(^�‡›Ÿ£½ÔæÿTF¶ïÃ2ó.Z»G]~ˆÍæ1Pfá5žÁó\˜ÔãêøZ¾å	'	
2
Z
¯
ÿ
Ö'9\d’'ä‹¶ÇÓ³Û3]js�£¾ül¯½ÆÕòQdh}wƒ¢œ²¡­Òë:KX…ç2JW^ðX�£æ$TFøf^ € ë :—Þ”ï–Ôñ	¼
fGRV¨ðLêwˆÓ¬ÞÝ¤é7	s
•¿jlr¾ŒEGbÍ›D üÔ:×‚²Áù’®¿�H	l	ü	1mpzýH�_eŠ�™á!©&)múK# a ß <ekü~
d„½Ì.?JTÑu©®¼ñÜpE d R^‡Ú	+”ýXnzÙïLrŸê	/†Ê¯èñÕ)¹=	I	�	·	ê	ý	‘
Æ
Û� 7iŽ¹Å8·x®¸	‰Øã+rž¢4`ÅDJPfÅù{*]‹·ÃÖånÑær�ÿìP�‡ÂÇ6—là|”¸ZFA»Ê  &‰     }  ‹        ‹7»IÖã¸cÍ’”ô›Žþ:"(³„’3U©NuÔ5‡ÇZ6[Ž�)ª3Ž³œFg2‡hi¡ä¦–ImiÀ-¤­“öf¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M»ƒ—‘÷Žž›¥œ{y�ýNL©^”è'ÄìÞKÒ �k�½7H0ó±Õ^Y´AF ]å´…jDÕJZ¼A¦?*Wó†Óìx�®J==¸¢£þÿÈúúàP�{÷‹êùeXAZ6Í¢á$"Úæ¸  AÓP€QÖMŽ cæ¸CEôàä ›ýÌþ$Ã6”Ì0")ÌÓ!ó�èôÏäÏÎú³«¯ëêì÷+<:¿ËS¹[ ;ú�+Æ¤Ž§ûõUèi+rHlþ*¬þ¡Ê½ÊêY {¢½^×{_æì.kª™@j�°ù ¥U„á©uV¥ÌZZâˆ‹J@ˆ/ô;ý44RcTØÛµ¤´×Ðˆ/ô;ý44RcTØÛµ¤´×Ù/Ïù{ÏÛóþ^óô¯?k¼ÿ`áåªªjcD<CÏ¸û„æ½’Cª Cä:£UNüè™½ªÁ¸mDtA²¬Øh]F�Í
ï:CšÃ˜x×G[;†<ò+Ã‹LÐü+ª‹Qñø¼#øX^4CLÃ› à÷¯£4â;¢ÞÀP9 1Hðï‹nß£¾<EI½¶Ö0Ã�œèo~?k}LWlÛr{<.‹A†àqDÛ@;3ÓÅâ³ÐíC3cñÃnË!ýƒøèñfØ UƒÎ«�­±â Áó›AÙ2ƒŸÈfŽNƒX:û#©Âló¿Í3lúæÏ®‡ºá0à
Åañõ1Ï×ãÛq±¯|óë˜T(°[*¼<8o½3 nØàJë?×¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸™òÇîNDè2[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘EÒj	¸Îã…=)úÁU‚Nèo]_|à“
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{gºú«Y¦…6¦Ð)¢í„Á�š…‰©Y]Y ¯3v¯ñT�~±Á°»ñ¿æ      !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ø<?xml version="1.0"?><Features nextValidID="17"><Feature recentID="16" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3352-3368" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3328-3344" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="830-848" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3421-3442" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3376-3382" color="#ffffff" type="standard"/><Segment range="3383-3400" color="#ffffff" type="standard"/><Segment name="-35" range="3401-3406" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5347-5451" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3158-3279" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1974-2109" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="582-785" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="bGH poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1082-1306" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1794-2123" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="202-581" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1352-1780" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3730-4315" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="NeoR/KanR" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2190-2984" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5277" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4486-5277" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5278-5346" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="3xMyc" directionality="1" translationMW="3573.98" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1050" maxFusedRunOn="1050" detectionMode="exactProteinMatch"><Segment range="958-1047" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;3 tandem Myc epitope tags&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>ca2c4198-5084-4847-b11e-dfd699f4c027</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="6:2:9">2018.12.12</Created>
<LastModified UTC="2:7:43">2023.6.27</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  *—      |  ˆ        ˆ7»IÖã¸cÍ’”ô›Žþ:"(³„’3U©NuÔ5‡ÇZ6[Ž�)ª3Ž³œFg2‡hi¡ä¦–ImiÀ-¤­“öf¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M»ƒ—‘÷Žž›¥œ{y�ýNL©^”è'ÄìÞKÒ �k�½7H0ó±'öqÉøi�zo¦Ÿ†˜äæúa‹ôyÍ„Ê•üá´û#ë’�On(¨ÿ¿ò>¾‡ø#Þýâú¾YVV�³h¸I¶¹®  ÐtÔ A u“Cc€Â¹ƒ®ÁQ}8¹ &ÿs?‰0Í¥3‚
s4È|çz=3ù3ó¾¬êëúú»=ÊÏ¯òÔîVÀþ¤
ñ©#©Ã~ÇýUz@†
Ü’Û?†G
«?¨r�/rº–Hè¯W�uÞ×ù»šê†¦P£Æ¬>h)Ua8j]U©s–¸�b"Ò�"KýÿM˜Õ66íi-5ô"KýÿM˜Õ66íi-5öKóþ^óöüÿ—¼ý+ÏÚï?Ø8yjªš˜Ñóî>á9¯E$„ê¨ù¨ÕS¿:&oj°nQl«6Q�£sB»ÃÎ€�æ°æuÑÖÃŽá�<ŠðâÓ4?
ê¢Ô|~/þ�Ó0æÈ8=Ç+èÍ8ŽÇ(·…0ƒŽhR<A»âÛ·èï�Romƒ†5Œ0ãg:ÃŸ�ÚÇßSÛ6ÄÄÜžÁ�O¢ÆÐa¸Q6ÐÌôñx¬ô;PÌØüpÄ²È`Æ>:<Y¶`óªàklxƒÃ€ˆ0|æÐvL çò£… Ã‡Î¾Èêp›<ïÆ³LÛ>¹³ë¡Ä®¸L¸±X|}LsõøöÜF¬kß<úæ
¬Ê¯ÏïLÀ¶8ºÏßõè©£N™`§VTÖM·V­Õ•6ª­ãk’ˆ*/W¶Ö&é¶.&|±û“‘:–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äQtš‚n3¸áOJ~°UD “ºÃWWß8$Âª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…žÙî¾ªÖi¡M©´
h»a0gf¡bjEVWV@+ÌÝ«üU#ß‡,plnüoù‚      !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  "<?xml version="1.0"?><Features nextValidID="19"><Feature recentID="18" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5274" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4483-5274" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5275-5343" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="17" name="NeoR/KanR" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2187-2981" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="16" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3727-4312" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1349-1777" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="202-581" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1791-2120" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="bGH poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1079-1303" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="582-785" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1971-2106" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3155-3276" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5344-5448" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3373-3379" color="#ffffff" type="standard"/><Segment range="3380-3397" color="#ffffff" type="standard"/><Segment name="-35" range="3398-3403" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1047" maxFusedRunOn="1047" detectionMode="exactProteinMatch"><Segment range="958-984" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="5" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1047" maxFusedRunOn="1047" detectionMode="exactProteinMatch"><Segment range="988-1014" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="4" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1047" maxFusedRunOn="1047" detectionMode="exactProteinMatch"><Segment range="1018-1044" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="3" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3418-3439" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="830-848" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3325-3341" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3349-3365" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  @<Notes>
<UUID>76a2b7e2-7c4b-4b1d-86f7-ef0923c7451f</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="6:2:9">2018.12.12</Created>
<LastModified UTC="2:15:18">2020.4.17</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  	 ý7zXZ  æÖ´F !   t/å£à@í¾] Ë‡ØÎf‘ƒÊý{3Ôé·Ú(1v%f M*	mj÷)p80çÌ*iÚ	´NY]Qó“�úF7î&ƒÕ!Ò÷ÀnÉ;_x’Ó¤è‡}	Q@)‰&ýøDhJ3\îFLšuÑ5ýb_íÖ]�~)ì-²štxú£ÅMê·�]Ÿw”µu;·å’9q%¼·×ÊP¹¨ãå ZBËûÿæ—Ô¹²å³óYoŽ×Þ�‡ÐÔLò \‡ÌÓÅ.$[«õò/‘e[ç !Ôpz‹&!,	^�‡t8ðé×Ðz0Ô²çµé2JÜ º@eLÔ�þ{`	ªU-†‘Z¤^$‹êJºŸÎ*ð½úgó’áHuŸÈ\w¨9<åJÿº�°ýOj/ÿ<_ù‘#ýÒÎHúLõ€lÝøô©dCÚ¶”ë«3yçP­”ŠHy=¿'œ¾¬;Ø+ul0ŽÔaì¯èKN¯æÏùëz¾oðÖãðÖ2ë»~ñC	ÎšËi¬â¤äÓ|ƒe[øl�Š7(ÄQ€øÖf8ªÍ�,a�esèvêŠ—­SÆyGS¹ÄÉô®žWXBóW¾Q~�î68ý¾Ø<Ò48býÕHÒ¦¤ÁsÇUD®‰eu×¯Óur5L—Š¥¢4Û#\ÚÎ*óc³éþÒmÊ¨9ìñßÕMä¯8î'� =’%Tî·‘ L««'T�`ßWÚÀl“(Ì~>]ŽË¿gíms˜iWž²L¶2Ê©©±ãÈ0™–AáË2½ŒÒ)vqaá7+F£Š}]¬Ài¸Æ?5Í¬S‡*Zƒ�y¾DsÅÄ¨.\ì.Ï.ÆiÐ,;$Óù=AÞ\bm½t8.ÐÑº9Ú}<µ=½ñÖ£`)P¹ä+}±Jþ„‚‚ßIÑ“õ.xõ+Ûe	©ÝñÑ‰©GŠR�$Mƒn\4øú{ÊU•‘5Œµx[¸Y¢ìb¼9¤�j^[xp•1û%Š%K�Ë3ëæÚÄšƒ¬¸èDd[ó¦²ÐÚ÷[�v¹‰6Í‹µ—7¿=aH}ðÑ}5µÕþ¼Öˆ*û’É{ûµçöÊóHþöêµVcaº“\áHûw,•ä:µ;©“‘’)ÂøF›KÖégbÞê°›¶ž$/²š…qC({ùœ³3�…Qb>XDÄŽ_µd]‰ú  §�t“�È¢®y>ó­Lô*Ì4u^U»G¥ Ì“áB ¯1PÁéY³Þ_†mÕÇí–éüþê–åÑX™1¨º§„2¡?f8ehÑw¦UÍ%K¬Í�dÍQ7PGÇDú]*ÐQË£²|ª‰pEw½o!¤Ã6@C­ ¤éSNöð¦Iüåù¸G4Š­Ž‰³&ö®ýv/˜öãÇV÷:Íw
|;Hº›R]ì÷�”³Uê¼HŽáeM_�šnû$àB8üø¨1”œic¢v ÆµwlFËû
µ[Õ@;â7d+®’ÜUvi³Oæcj†ûº|*!·6¤È½@ÁïÄ|òó—-¹k1OJ“ŠÊÐ•™ø¡oùOßmdŽàË5NÃ´#I8RÃöÄ{Û¥®[›+š`©GÁBVñbƒE™G”O¿å¡©©VB6¦îPÌAìF½š×µé#}¤‡ƒÂÛsü.Íg¹�ß!B¬Ÿï¤É~“b�‡î%�Ñf8Û+,/“¨Ž%6BlX•{þÛ®¿Ç>«%@ÜÕü+|‡ô&6ââ ë>¹^ÙW—º@;—ÈPàÆ„TŽþ²Ê±˜õY¥â<–eÑ!l«’ Ÿ[Ê/3VIoó@—&1tÉpåájè£ŸùàU�·£•ã{ZØ?H?Ÿ»äö‚ZúË¡@Up¢m·`²µ²íHÿ/÷:5·­‡Ð¯ÇKØªàhôlpYÜS§ÁÃ�Ù17I¡;·¶�ñ>Þ¯Ùé,D%`²1íí‚ÕcŠ™Ìæœ¿É>šó©km"­<•RJ¿Ú’‚ŠÖ‘:8ØßÛ�Ãý¤zþ5ßT$öþ+ aø_9b$ñ7Þæf›6Ÿ+xO/bHH.#úíðÌ¹¢

Üzob‘W0eÒÓIÞg‹>ôƒbâLsbÿ"’ž¿©<¿.º_Eu}¼Î2GS-r¸!ÅGyˆ�ïki`ßÕîé+®2nouá˜uÈÑƒÌBuÒ³§‰x.ˆ�‚nKÔû ó¦}ÏAë�ÝCâ6ÈÎ.œ)QÚ/ü.�j–Ú9’RÐR#›¤•»€Ðâ>‘Gï)½<­§0qt¬�:sW3óæaB[yw!gß*X—âÂEßxh%¯q`_æHS:±‹™5¬àªãïÏ™3gÛK™jÚ^•˜Âê¦ì»kšQf¥ø¹%iâBðO.`­x?ÜSŽŒIp*+Ïü`ª}ÍÃ»M*š°g†FÐá„¥öÐýF	øL?áÐb€ð)SGÆÂ¹™$nÖ ÂÝµ‰d‹¾Šy‡A7™*LûøG�eJÍ‡½ÿ³€†Ä
Nàv�¬€Gä¾"¬À�™kÿ!˜&tã(¡>ßßwå~�\ö;N¬Ê�&AAóâ§ÓÓBcG_g¦ó]¯MtîušÚ~� Ø�[¹°)ž}o¬Ss‰áu´“çOP;D¹{Ø]jÇU±•“š Ï¿ëçe1÷“0ì¬|c7BœÀˆNðœ‰PFZ êÔí&dw»
½l¬¡Fû-³! “�ù™~¸9‹øÃpÅ�I\�ã?é¡– Øk&�öUuIÎ(¾µîÜE+ôEbóß]tó¿|~äFƒÖ:gæ\&]ö¤6PµèÖC£v/Ó8ö¼›ËƒÂ ³8êÊ÷º[ýóÜå„i1PëÑ=Vx¾Ñú	³TÐ'‚Äß§?Ó
þò¦Z¡‡*ï¨Áœw)Y>†=áh]H€~�-Ô7U~¡ÇA©?ÒöÄœµ$ÉSæ     Ï/«}/(WŠ Úî� [#<â±Ägû    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  (ø<?xml version="1.0"?><Features nextValidID="20"><Feature recentID="0" name="3xMyc" directionality="1" translationMW="3573.98" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2940" maxFusedRunOn="2940" detectionMode="exactProteinMatch"><Segment range="2848-2937" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;3 tandem Myc epitope tags&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="EQKLISEEDLEQKLISEEDLEQKLISEEDL"/></Q></Feature><Feature recentID="1" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="7167" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="6376-7167" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="7168-7236" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="2" name="NeoR/KanR" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4080-4874" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5620-6205" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3242-3670" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="202-581" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3684-4013" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="bGH poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2972-3196" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="582-785" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3864-3999" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5048-5169" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7237-7341" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="5266-5272" color="#ffffff" type="standard"/><Segment range="5273-5290" color="#ffffff" type="standard"/><Segment name="-35" range="5291-5296" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5311-5332" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="830-848" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5218-5234" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5242-5258" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="925-934" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="18" name="Kozak sequence" type="regulatory" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="929-938" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="19" name="PRMT5" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2940" maxFusedRunOn="2940"><Segment range="931-2841" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MAAMAVGGAGGSRVSSGRDLNCVPEIADTLGAVAKQGFDFLCMPVFHPRFKREFIQEPAKNRPGPQTRSDLLLSGRDWNTLIVGKLSPWIRPDSKVEKIRRNSEAAMLQELNFGAYLGLPAFLLPLNQEDNTNLARVLTNHIHTGHHSSMFWMRVPLVAPEDLRDDIIENAPTTHTEEYSGEEKTWMWWHNFRTLCDYSKRIAVALEIGADLPSNHVIDRWLGEPIKAAILPTSIFLTNKKGFPVLSKMHQRLIFRLLKLEVQFIITGTNHHSEKEFCSYLQYLEYLSQNRPPPNAYELFAKGYEDYLQSPLQPLMDNLESQTYEVFEKDPIKYSQYQQAIYKCLLDRVPEEEKDTNVQVLMVLGAGRGPLVNASLRAAKQADRRIKLYAVEKNPNAVVTLENWQFEEWGSQVTVVSSDMREWVAPEKADIIVSELLGSFADNELSPECLDGAQHFLKDDGVSIPGEYTSFLAPISSSKLYNEVRACREKDRDPEAQFEMPYVVRLHNFHQLSAPQPCFTFSHPNRDPMIDNNRYCTLEFPVEVNTVLHGFAGYFETVLYQDITLSIRPETHSPGMFSWFPILFPIKQPITVREGQTICVRFWRCSNSKKVWYEWAVTAPVCSAIHNPTGRSYTIGL"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ¬<Notes>
<UUID>39b58435-05b3-4a0d-8593-c933e69d1148</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pCMV-PRMT5(human)-3Ã—Myc-BGH-Neo</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="6:2:9">2018.12.12</Created>
<LastModified UTC="22:27:16">2025.9.20</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         
?????                                                                                                       ÿ d      HO  d               E              ÿÿÿÿYô? P                                                                                                                                                              3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
